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PASCUAL , Alberto

Last name: PASCUAL

First name: Alberto

January 31, 2018 (v1)

Publication

Metadata-only

Substrate binding and catalysis by ribonuclease P from cyanobacteria and Escherichia coli are affected differently by the 3′ terminal CCA in tRNA precursors

Pascual, Alberto Vioque Peña, Agustín

We have studied the effect of the 3′ terminal CCA sequence in precursors of tRNAs on catalysis by the RNase P RNA or the holoenzyme from the cyanobacterium Synechocystis sp. PCC 6803 in a completely homologous system. We have found that the absence of the 3′ terminal CCA is not detrimental to activity, which is in sharp contrast to what is...

Uploaded on: December 5, 2022
July 25, 2017 (v1)

Publication

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Cloning and expression of the algL gene, encoding the Azotobacter chroococcum alginate lyase: Purification and characterization of the enzyme

Peciña, Ana Pascual, Alberto Paneque Guerrero, Antonio

The alginate lyase-encoding gene (algL) of Azotobacter chroococcum was localized to a 3.1-kb EcoRI DNA fragment that revealed an open reading frame of 1,116 bp. This open reading frame encodes a protein of 42.98 kDa, in agreement with the value previously reported by us for this protein. The deduced protein has a potential N-terminal signal...

Uploaded on: December 5, 2022