PRADO GOTOR , Rafael

El trabajo que a continuación se presenta es un intento de realizar un estudio de los efectos de los campos eléctricos sobre la cinética de las reacciones químicas. En particular, nos interesaba estudiar los efectos del campo eléctrico en el proceso de transferencia electrónica que se produce en el complejo binuclear en el que un electrón se...

The biological activity of nucleic acids not only depends on the thermodynamic properties of DNA-ligand complexes. It can be conditioned by the kinetics of formation of these complexes. Therefore, it seemed interesting to address the thermodynamic and kinetic study of the interactions of DNA with nanoparticles acting as small ligands. Between...

La presente solicitud de patente se refiere a un método calorimétrico y kit para la detección y cuantificación de receptores aniónicos de alta carga, como ADN de doble cadena y micelas aniónicas de SDS, mediante agregados de nanopartículas de oro protegid

Procedimiento para la detección colorimétrica de lisozima en orina por nanopartículas agregadas de oro. La presente invención tiene por objeto un procedimiento que permite detectar lisozima en orina, empleando nanopartículas de oro protegidas con iones ci

The binding of gold nanoparticles capped with N-(2-mercaptopropionyl)glycine (Au@tiopronin) with double-stranded DNA has been investigated and quantified in terms of free energies by using two different approaches. The first approach follows the DNA conformational changes induced by gold nanoparticles using the CD technique. The second...

Procedimiento para la detección colorimétrica de lisozima por nanopartículas agregadas de oro. La presente invención tiene por objeto un procedimiento que permite detectar lisozima en disolución acuosa, añadiendo nanopartículas de oro protegidas con iones

Objetivos: Se han detectado claras deficiencias en el proceso de enseñanza-aprendizaje en las prácticas de la asignatura de "Física Aplicada a Ciencias de la Salud" del primer curso del Grado en Farmacia. Por tanto, el objetivo de este trabajo es desarrollar un sencillo programa de evaluación que permita extraer información inmediata sobre la...

Potential NLO-phore materials based on chitosan are described for the first time in this study. A series of fluorescent and quaternized pyridinium– chitosan derivatives have been synthesized by reaction of this polymer with easily available tunable pyrylium tetra fluoroborate salts. Among other spectroscopic techniques, 19 F NMR, 13 C CPMAS...

Surfactant-based nanostructures are promising materials for designing novel colorimetric biosensors based on aggregation/disaggregation phenomena. In this work, a colorimetric sensor based on the plasmonic shift of surfactant-capped gold nanoparticles via the disaggregation mechanism was developed. To perform this, the optimum SDS concentration...

Due to their chemical stability, high biocompatibility, excellent structural, optical, magnetic and catalytic properties, gold nanoparticles (AuNPs) have been widely used as therapeutics, delivery agents and transfection vectors. Since successful therapy for curing cancer, and other genetic diseases, requires the transport of DNA into the cell...

The development of a new method for covalent immobilization of biomolecules on the surface of bare gold nanoparticles (AuNPs) by using crosslinking agents in one step is presented. A very compatible biopolymer such as chitosan has been used as a target molecule to probe the viability of the proposed methodology. Click chemistry, based on...

We decorated a single giant DNA (1.66 × 105 base pairs) with gold nanoparticles through the simple procedure of mild warming, without denaturation of the DNA molecule. Single-molecule observation with fluorescence microscopy revealed that individual decorated DNA molecules stay in the bulk solution by avoiding aggregation and precipitation, and...

We explore the possibilities of the CIELab chromaticity system parameters' evolution in order to study the interaction between two non-colored biological reactants, lysozyme and adenosine, and the red-colored, non-functionalized citrate gold nanoparticles. By measuring and quantifying the AuNP color changes, which are strongly dependant on...

The stabilizing effect of lysozymes to salt addition over a gold colloid are exploited in order to detect lysozymes in human urine samples. The present research is aimed at the development of a fast, naked-eye detection test for urinary lysozymuria, in which direct comparison with a colorimetric reference, allows for the immediate determination...

The use of silver nanoparticles stabilized with citrate and polyvinylpyrrolidone as a sensor for aluminum ions determination is proposed in this paper. These non-functionalized and specific nanoparticles provide a highly selective and sensitive detection system for aluminum in acidic solutions. The synthesized nanoparticles were characterized...

Stable oleuropein-coated gold nanoparticles in aqueous media were synthesized for the first time. Oleuropein (OLE) concentration in the reaction medium was found to greatly influence the outcome and stability of the resulting nanocolloid, with a marked decrease in particle size being found for the more concentrated oleuropein solutions. The...

The DNA conformational changes induced by different members of the N,N0-bis(dimethyldodecyl)-a-ualkanediammonium dibromide series (m-s-m, m ¼ 12, s ¼ 3 and 6) and the analogous series of hexadecyl gemini surfactants (m ¼ 16, s ¼ 3 and 6) were investigated in aqueous media by means of circular dichroism (CD), zeta potential, dynamic light...