El ADN sufre muchísimos tipos de daño debido a fuentes externas o internas. No obstante, los cortes de doble cadena son de los más peligrosos porque si no se reparan adecuadamente, pueden conducir a mutaciones e inestabilidad genómica. Para reparar este daño, las células han desarrollado principalmente dos vías: la recombinación homóloga o la...
-
February 20, 2020 (v1)PublicationUploaded on: December 2, 2022
-
January 10, 2023 (v1)Publication
In order to survive to the exposure of DNA damaging agents, cells activate a complex response that coordinates the cellular metabolism, cell cycle progression, and DNA repair. Among many other events, recent evidence has described global changes in mRNA splicing in cells treated with genotoxic agents. Here, we explore further this DNA...
Uploaded on: March 3, 2023 -
May 16, 2018 (v1)Publication
The DNA damage response (DDR) is an extensive signaling network that is robustly mobilized by DNA double-strand breaks (DSBs). The primary transducer of the DSB response is the protein kinase, ataxia-telangiectasia, mutated (ATM). Here, we establish nuclear poly(A)-binding protein 1 (PABPN1) as a novel target of ATM and a crucial player in the...
Uploaded on: December 4, 2022 -
March 15, 2018 (v1)Publication
BRCA1 is a tumor suppressor that regulates DNA repair by homologous recombination. Germline mutations in BRCA1 are associated with increased risk of breast and ovarian cancer and BRCA1 deficient tumors are exquisitely sensitive to poly (ADP-ribose) polymerase (PARP) inhibitors. Therefore, uncovering additional components of this DNA repair...
Uploaded on: December 4, 2022 -
September 7, 2023 (v1)Publication
The centrosome is a cytoplasmic organelle with roles in microtu-bule organization that has also been proposed to act as a hub forcellular signaling. Some centrosomal components are required forfull activation of the DNA damage response. However, whether thecentrosome regulates specific DNA repair pathways is not known.Here, we show that...
Uploaded on: October 18, 2023 -
May 28, 2019 (v1)Publication
The exosome is a ribonucleolytic complex that plays important roles in RNA metabolism. Here we show that the exosome is necessary for the repair of DNA double-strand breaks (DSBs) in human cells and that RNA clearance is an essential step in homologous recombination. Transcription of DSB-flanking sequences results in the production of...
Uploaded on: December 4, 2022 -
April 16, 2019 (v1)Publication
DNA breaks are complex lesions that can be repaired either by non-homologous end joining (NHEJ) or by homologous recombination (HR). The decision between these two routes of DNA repair is a key point of the DNA damage response (DDR) that is controlled by DNA resection. The core machinery catalyzing the resection process is well established....
Uploaded on: December 4, 2022 -
September 21, 2021 (v1)Publication
The maintenance of genomic stability requires the coordination of multiple cellular tasks upon the appearance of DNA lesions. RNA editing, the post-transcriptional sequence alteration of RNA, has a profound effect on cell homeostasis, but its implication in the response to DNA damage was not previously explored. Here we show that, in response...
Uploaded on: December 4, 2022