En esta Tesis se ha abordado el estudio de la regulación de la ruta biosintética de la Treonina en la levadura Saccharomyces cerevisiae. Para ello se han utilizado dos estrategias diferentes: 1. Caracterización de algunas enzimas de esta ruta: Se ha puesto a punto un método para la medida de las actividades homoserina quinasa y aspartato...
-
November 27, 2014 (v1)PublicationUploaded on: March 27, 2023
-
April 21, 2016 (v1)Publication
In this work, we isolated and characterized mutants that overproduce threonine from Saccharomyces cerevisiae. The mutants were selected for resistance to the threonine analog a-amino-13-hydroxynorvalerate (hydroxynorvaline), and, of these, the ones able to excrete threonine to the medium were chosen. The mutant strains produce between 15 and 30 ...
Uploaded on: December 5, 2022