SAGGAU , Peter

Fast noninvasive three-dimensional (3D) imaging is crucial for quantitatively studying highly dynamic events ranging from flow cytometry to developmental biology. Light-sheet microscopy has emerged as the tool-of-choice for 3D characterization of rapidly evolving systems. However, to obtain a 3D image, either the sample or parts of the...

Light-sheet microscopes with an extended depth of field (EDOF) offer a simple but powerful route toward fast volumetric imaging. However, methods for EDOF typically result in a loss of signal-to-noise ratio. Here, we propose a parallelization strategy as a simple solution. By illuminating multiple acoustically generated light sheets at...

We propose a novel light-sheet microscope, capable of high-speed volumetric imaging with high contrast and signal-to-noise ratio. The working principle is founded on extending the depth-of-field and enconding the illumination with multiple light-sheets.