SCIPIONI , Lorenzo

In this work we will present our current advances in the development of the CCA (Comprehensive Correlation Analysis) technique using the Zeiss Airyscan detector. This detector consists of 32 GaAsP PMT arranged in a hexagonal pattern, featuring a fast temporal sampling (down to 1.28 μs per frame). It can also be used in a super-resolution...

Tuesday, February 20, 2018 533a accessibility, spatiotemporal confinement and oligomerization states from a single analysis of a few seconds in living cells. The combination of fast sampling speed (down to 1.23 μs per frame) and super-resolution capability opens the doors to the investigation of spatiotemporal dynamics that are not accessible...

Raster image correlation spectroscopy (RICS) is a powerful method for measuring molecular diffusion in live cells directly from images acquired on a laser scanning microscope. However, RICS only provides single average diffusion coefficients from regions with a lateral size on the order of few micrometers, which means that its spatial...

The precise localization of nanometric objects in three dimensions is essential to identify functional diffusion mechanisms in complex systems at the cellular or molecular level. However, most optical methods can achieve high temporal resolution and high localization precision only in two dimensions or over a limited axial (z) range. Here we...

The observation of molecular diffusion at different spatial scales, and in particular below the optical diffraction limit (<200 nm), can reveal details of the subcellular topology and its functional organization. Stimulated-emission depletion microscopy (STED) has been previously combined with fluorescence correlation spectroscopy (FCS) to...

Defects in the global organization of chromatin are relevant in many pathological processes, including cancer. The activation of oncogenes has been recognized as a triggering event of alterations in the dynamics of replication, transcription and DNA damage response (DDR), that may lead to increased DNA damage and the onset of genomic...

The fact that the size of many molecular complexes is in the range of 10 to 100nm has generated a growing interest for the development of super-resolved fluorescence microscopy techniques. Optical super-resolution is generally achieved by switching on-off the fluorophores by stochastic or targeted approaches [1]. For instance, in stimulated...