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STERVBO , Ulrik

Last name: STERVBO

First name: Ulrik

2022 (v1)

Publication

Metadata-only

Bulk gDNA Sequencing of Antibody Heavy-Chain Gene Rearrangements for Detection and Analysis of B-Cell Clone Distribution: A Method by the AIRR Community

Rosenfeld, Aaron M Meng, Wenzhao Horne, Kalisse I

In this method we illustrate how to amplify, sequence, and analyze antibody/immunoglobulin (IG) heavy-chain gene rearrangements from genomic DNA that is derived from bulk populations of cells by next-generation sequencing (NGS). We focus on human source material and illustrate how bulk gDNA-based sequencing can be used to examine clonal...

Uploaded on: January 31, 2024