TRIANNI , Alberta

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Label free microscopic approaches have become a necessity to explore, image, and understand, complex biological systems with minimal sample modifications [1]. These innovative microscopes exploit the native photophysical processes present when light interacts with matter, presenting a plethora of options not only for introducing image contrast,...

180a Wednesday, February 24, 2021 ptychographic module was developed and mounted on a commercial SIM, to simultaneously collect ptychography and SIM data. We show how changes in the morphology of a HGPS nucleus affect the phase compared to normal cells, and how the SIM-ptychography approach can increase the resolution compared with conventional...

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Polarized light scanning microscopy is a non-invasive and contrast-enhancing technique to investigate anisotropic specimens and chiral organizations. However, such arrangements suffer from insensitivity to confined blend of structures at sub-diffraction level. Here for the first time, we present that the pixel-by-pixel polarization modulation...

Light-sheet microscopes with an extended depth of field (EDOF) offer a simple but powerful route toward fast volumetric imaging. However, methods for EDOF typically result in a loss of signal-to-noise ratio. Here, we propose a parallelization strategy as a simple solution. By illuminating multiple acoustically generated light sheets at...

We propose a novel light-sheet microscope, capable of high-speed volumetric imaging with high contrast and signal-to-noise ratio. The working principle is founded on extending the depth-of-field and enconding the illumination with multiple light-sheets.