VICIDOMINI , Giuseppe

One of the key frontiers in optical imaging is to maximize the spatial information retrieved from a sample while minimizing acquisition time. Confocal laser scanning microscopy is a powerful imaging modality that allows real-time and high-resolution acquisition of two-dimensional (2D) sections. However, in order to obtain information from...

Confocal laser scanning (CLS) and two-photon excitation (TPE) microscopy are powerful techniques for 3D imaging of biological samples. Although CLS and TPE microscopy images are better than standard epifluorescence images, they still undergo degradation due to blurring and random noise because of the inherent nature of the physical phenomenon...

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There is growing interest in techniques based on using detector arrays in confocal microscopy [1]. Many different implementations have been proposed, and various different names given to these techniques, one such name being image scanning microscopy [2]. The images from the elements of the detector array can be combined optically [3], but more...

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The quantitative analysis of fluorescence perturbation experiments such as fluorescence recovery after photobleaching (FRAP) requires suitable analytical models to be developed. When diffusion in 31) environments is considered, the description of the initial condition produced by the perturbation (i.e., the photobleaching of a selected region...