VARGAS MUÑOZ , María De Los Ángeles

La nitrato reductasa purificada de hojas de espinaca en presencia de un reductor adecuado se inactiva de forma específica por acetileno. Dicha inactivación afecta solo a su actividad terminal. El sitio de acción del acetileno en la enzima parece estar estrechamente relacionado con el sitio activo para el nitrato. La nitrato reductasa...

Nitrate reductase from spinach (Spinacia oleracea L.) leaves, which had been inactivated in vitro by incubation with N A D H and cyanide, was fully reactivated in minutes when irradiated in anaerobic conditions with red light in the presence o f m ethylene blue. Both the rate and the extent of reactivation increased with light intensity ( 6 to...

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The isolation and characterization of the phytoene synthase gene from the green microalga Chlorella zofingiensis (CzPSY), involved in the first step of the carotenoids biosynthetic pathway, have been performed. CzPSY gene encodes a polypeptide of 420 amino acids. A single copy of CzPSY has been found in C. zofingiensis by Southern blot...

The lack of highly active endogenous promoters to drive the expression of transgenes is one of the main drawbacks to achieving efficient transformation of many microalgal species. Using the model chlorophyte Chlamydomonas reinhardtii and the paromomycin resistance APHVIII gene from Streptomyces rimosus as a marker, we have demonstrated that...

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