VICIDOMINI , G.

In stimulated emission depletion (STED) microscopy, the role of the STED beam is to de-excite, via stimulated emission, the fluorophores that have been previously excited by the excitation beam. This condition, together with specific beam intensity distributions, allows obtaining true sub-diffraction spatial resolution images. However, if the...

Method for operating a point laser- scanning microscope, comprising scanning a sample with a focused illumination laser beam (EB); recording, by means of an array of detecting elements (23), a plurality of images of the sample over a scan by the laser beam, wherein the detecting elements (23) are configurable to an intensity mode, in which the...