VICIDOMINI , Giuseppe

Stimulated emission depletion (STED) microscopy provides subdiffraction resolution while preserving useful aspects of fluorescence microscopy, such as optical sectioning, and molecular specificity and sensitivity. However, sophisticated microscopy architectures and high illumination intensities have limited STED microscopy's widespread use in...

Precise knowledge of the effective spatial resolution in a stimulated emission depletion (STED) microscopy experiment is essential for reliable interpretation of the imaging results. STED microscopy theoretically provides molecular resolution, but practically different factors limit its resolution. Because these factors are related to both the...

Stimulated emission depletion (STED) microscopy based on time-gated detection is a straightforward implementation, which substantial reduces the STED beam (peak) intensity to obtain effective sub-diffraction imaging. Furthermore, when combined with STED beam operating in continuous-wave (CW) lowers the complexity and the cost with respect to...

Raster image correlation spectroscopy (RICS) is a powerful method for measuring molecular diffusion in live cells directly from images acquired on a laser scanning microscope. However, RICS only provides single average diffusion coefficients from regions with a lateral size on the order of few micrometers, which means that its spatial...