VILLALOBO POLO , Eduardo

Calcium (Ca2+) is a universal second messenger that plays a key role in cellular signaling. However, Ca2+ signals are transduced with the help of Ca2+-binding proteins, which serve as sensors, transducers, and elicitors. Among the collection of these Ca2+-binding proteins, calmodulin (CaM) emerged as the prototypical model in eukaryotic cells....

En el siglo XIX es cuando se producen los avances en microbiología alimentaria, es decir, de aquella parte de la microbiología cuyo objeto de estudio son los microorganismos de los alimentos. Posiblemente, Pasteur sea el gran impulsor de esa especialidad de la microbiología, porque gracias a sus estudios sobre fermentación y putrefacción de...

The Ca2+-sensor protein calmodulin (CaM) is a major regulator of multiple cell functions. A unique and puzzling feature of human, and all so far investigated mammals, is the presence of three distinct CaM genes on different chromosomes, which code for identical proteins. How this case of apparent genetic redundancy evolved and why it could be...

Motivación: ferrous iron oxidation can take place in partly controlled biological reactors inoculated with mixed cultures of acidophilic microorganisms. In these bioreactors, liquor pH can affect productivity. We aimed at determining whether productivity changes are directly due to the chemistry of iron...

The parasitic protozoan Entamoeba histolytica relies on a very dynamic cytoskeleton in order to invade and survive in host tissues. Identification of cytoskeletal elements is key to understanding these processes. Here we present the characterization of EhLimA, the first LTM protein of E. histolytica. EhLimA consists of a single LIM domain at...

We identified the Grb7 family members, Grb10 and Grb14, as Ca2+-dependent CaM-binding proteins using Ca2+-dependent CaM-affinity chromatography as we previously did with Grb7. The potential CaM-binding sites were identified and experimentally tested using fluorescent-labeled peptides corresponding to these sites. The apparent affinity constant...

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Actin is a cytoskeletal protein that is ubiquitous in eukaryotes, hence the corresponding genes and proteins have been isolated from numerous organisms as different as animals, plants, fungi and protozoa. Several atomic models are available for the monomeric as well as the filamentous form, and more than 70 proteins that bind actin and control...

The process of excystment of Sterkiella histriomuscorum (Ciliophora, Oxytrichidae) leads in a few hours, through a massive influx of water and the resorption of the cyst wall, from an undifferentiated resting cyst to a highly differentiated and dividing vegetative cell. While studying the nature of the genes involved in this process, we...

The flooded packed-bed bioreactor plays a major role in the field of applications of ferrous ion bio-oxidation. The pH is an important variable in the control of this type of reactor, upon which the functionality of biofilm depends. In the present work, five continuous flooded packed-bed reactors have been inoculated with mixed...

Se ha secuenciado un fragmento de un gen de la a-tubulina, amplificado por PCR, del cil iado hipotrico Histriculus cavicola. Este fragmento, de 1182 pb, contiene un codón de terminación (UAA) en la fase de lectura, que en otros ciliados hipotricos codifica la glutamina. La comparación de los genes de la cx-tubulina de varias clases de ciliados...

Ribosome synthesis is a complex process that involves a large set of protein trans-acting factors, among them DEx(D/H)-box helicases. These are enzymes that carry out remodelling activities onto RNAs by hydrolysing ATP. The nucleolar DEGD-box protein Dbp7 is required for the biogenesis of large 60S ribosomal subunits. Recently, we have shown...

In Saccharomyces cerevisiae, more than 250 trans-acting factors are involved in the maturation of 40S and 60S ribosomal subunits. The expression of most of these factors is transcriptionally coregulated to ensure correct ribosome production under a wide variety of environmental and intracellular conditions. Here, we identified the essential...

The objective of the present work was to evaluate novel active films made with biopolymeric matrices as carriers of a living Bdellovibrio bacteriovorus HD100 strain, a predatory bacterium with antimicrobial potentials against pathogens. Biopolymer films were prepared by a casting method using the following mixtures: collagen/sodium...

Synthesis of eukaryotic ribosomes involves the assembly and maturation of precursor particles (pre-ribosomal particles) containing ribosomal RNA (rRNA) precursors, ribosomal proteins (RPs) and a plethora of assembly factors (AFs). Formation of the earliest precursors of the 60S ribosomal subunit (pre-60S r-particle) is among the least...