ZUNINO , Alessandro

Bessel and annular laser beams offer intriguing possibilities for material processing. However, current beam shaping methods can be limited in tunability, speed, or parallelization possibilities. Here, we show how ultrasounds in liquids enable generating user-selectable arrays of Gaussian, Bessel-like, or annular beams. By cascading two...

Light-sheet microscopes with an extended depth of field (EDOF) offer a simple but powerful route toward fast volumetric imaging. However, methods for EDOF typically result in a loss of signal-to-noise ratio. Here, we propose a parallelization strategy as a simple solution. By illuminating multiple acoustically generated light sheets at...

We propose a novel light-sheet microscope, capable of high-speed volumetric imaging with high contrast and signal-to-noise ratio. The working principle is founded on extending the depth-of-field and enconding the illumination with multiple light-sheets.

The properties of signal strength and integrated intensity in a scanned imaging system are reviewed. These prop-erties are especially applied to confocal imaging systems, including image scanning microscopy. The integrated intensity, equal to the image of a uniform planar (sheet) object, rather than the peak of the point spread function, is a...

We discuss the effects of image scanning microscopy using doughnut beam illumination on the properties of signal strength and integrated intensity. Doughnut beam illumination can give better optical sectioning and background rejection than Airy disk illumination. The outer pixels of a detector array give a signal from defocused regions, so...

To date, the feasibility of super-resolution microscopy for imaging live and thick samples is still limited. Stimulated emission depletion (STED) microscopy requires high-intensity illumination to achieve sub-diffraction resolution, potentially introducing photodamage to live specimens. Moreover, the out-of-focus background may degrade the...