DE LIMA , Juliana Silva

Incorporating enzymes into calcium alginate beads is an effective method to immobilise them and to preserve, at the same time, their catalytic activity. Sodium alginate was mixed with Aspergillus ficuum tannase in aqueous solution, and tannase-loaded calcium alginate beads were prepared using a simple droplet-based microfluidic system....

Tannase (tannin acyl hydrolase, E.C. 3.1.1.20) is an enzyme that catalyzes the hydrolysis of ester and depside linkages in hydrolysable tannins such as tannic acid, releasing gallic acid and glucose. It has several commercial applications in food industry, among which are gallic acid production, reduction of tannin content in fruit juices, and...

Responsible for hydrolysis of tannins present in several beverages, tannase is an industrial enzyme of the great potential for applications in the food industry. Tannase was covalently immobilized onto magnetic nanoparticles composed of diatomaceous earth coated with polyaniline (mDE-PANI-TANNASE). This enzyme derivative showed 41% of residual...