Published 2014
| Version v1
Journal article
High levels of SOX5 decrease proliferative capacity of human B cells, but permit plasmablast differentiation
Creators
- Rakhmanov, Mirzokhid
- Sic, Heiko
- Kienzler, Anne-Kathrin
- Fischer, Beate
- Rizzi, Marta
- Seidl, Maximilian
- Melkaoui, Kerstina
- Unger, Susanne
- Moehle, Luisa
- Schmit, Nadine E.
- Deshmukh, Sachin D.
- Ayata, Cemil Korcan
- Schuh, Wolfgang
- Zhang, Zhibing
- François-Loïc, Cosset
- Verhoeyen, Els
- Peter, Hans-Hartmut
- Voll, Reinhard E.
- Salzer, Ulrich
- Eibel, Hermann
- Warnatz, Klaus
Contributors
Others:
- Center for Chronic Immunodeficiency (CCI), University Medical Center Freiburg, Freiburg, Germany ; Center for Chronic Immunodeficiency (CCI), University Medical Center Freiburg, Freiburg, Germany
- Universitäts Klinikum Freiburg = University Medical Center Freiburg (Uniklinik)
- Friedrich-Alexander Universität Erlangen-Nürnberg (FAU)
- Virginia Commonwealth University (VCU)
- Virus enveloppés, vecteurs et immunothérapie – Enveloped viruses, Vectors and Immuno-therapy (EVIR) ; Centre International de Recherche en Infectiologie - UMR (CIRI) ; École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL) ; Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL) ; Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)
- Centre méditerranéen de médecine moléculaire (C3M) ; Université Nice Sophia Antipolis (1965 - 2019) (UNS) ; COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Côte d'Azur (UCA)
Description
Currently very little is known about the differential expression and function of the transcription factor SOX5 during B cell maturation. We identified two new splice variants of SOX5 in human B cells, encoding the known L-SOX5B isoform and a new shorter isoform L-SOX5F. The SOX5 transcripts are highly expressed during late stages of B-cell differentiation, including atypical memory B cells, activated CD21low B cells and germinal center B cells of tonsils. In tonsillar sections SOX5 expression was predominantly polarized to centrocytes within the light zone. After in vitro stimulation, SOX5 expression was down-regulated during proliferation while high expression levels were permissible for plasmablast differentiation. Overexpression of L-SOX5F in human primary B lymphocytes resulted in reduced proliferation, less survival of CD138neg B cells, but comparable numbers of CD138+CD38hi plasmablasts compared to control cells. Thus, our findings describe for the first time a functional role of SOX5 during late B cell development reducing the proliferative capacity and thus potentially affecting the differentiation of B cells during the germinal center response.
Abstract
International audienceAdditional details
Identifiers
- URL
- https://hal.archives-ouvertes.fr/hal-01911351
- URN
- urn:oai:HAL:hal-01911351v1
Origin repository
- Origin repository
- UNICA