A Liquid Tunable Microscope as a New Paradigm in Optical Microscopy to Paint 4D Chromatin Organisation in the Cell Nucleus

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The possibility of integrating different light-matter interaction ways for producing images in optical microscopy is the starting point for the design and realization of a brand new optical microscope that has been named "liquid tunable microscope". This represents a new paradigm in data collection and image formation with a potential high impact in biophysics. It is designed with the aim of bringing a contribute to answer a key question in cellular and molecular biology: what are the local and global 4D (x, y, z, t) chromatin structures in the nucleus that rule the compaction and function of the human genome in the interphase cells and mitotic chromosomes? We aim to achieve: 1) the realization of a liquid microscope in the sense of allowing multiple contrasts available in the image formation process including a label-free one; 2) the painting, at single cell level, of a 4D quantitative vision of those …

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