Fodinicurvata halophila sp. nov., a moderately halophilic bacterium from a marine saltern

Description

A Gram-stain-negative, rod-shaped, facultatively anaerobic, moderately halophilic bacterium, designated strain BA45ALT, was isolated from water of a saltern located in Santa Pola, Alicante, Spain. Cells were motile, and catalase-and oxidase-positive. Strain BA45ALT grew at temperatures in the range 14-45 °C (optimally at 37 °C), at pH 5.0-9.0 (optimally at pH 7.5), and in media containing 5-20% (w/v) salts [optimally in media containing 10% (w/v) salts]. Phylogenetic analysis based on the comparison of 16S rRNA gene sequences revealed that strain BA45ALT is a member of the genus Fodinicurvata. The closest relatives to the novel strain were Fodinicurvata fenggangensis YIM D812T and Fodinicurvata sediminis YIM D82T with sequence similarities of 98.2% and 97.4%, respectively. DNA-DNA hybridization between the novel isolate and these phylogenetically related species revealed relatedness values of 30% and 15%, respectively, with respect to the aforementioned species. The major cellular fatty acids of strain BA45ALT were C18: 1ɷ7c, C16: 0 and iso-C15: 0. The G+C content of the genomic DNA of strain BA45ALT was 58.0 mol%, and the polar lipid pattern consisted of diphosphatidylglycerol, phosphatidylmethylethanolamine and a number of unknown phospholipids and lipids. Based on phenotypic, chemotaxonomic and phylogenetic data presented in this study, strain BA45ALT constituted a novel species of the genus Fodinicurvata, for which the name Fodinicurvata halophila sp. nov. is suggested. The type strain is BA45ALT (=CCM 8504T=CECT 8472T=JCM 19075T=LMG 27945T).

Abstract

Ministerio de Ciencia e Innovación CGL2013-46941-P, BIO2011-12879-E

Abstract

Junta de Andalucía P10-CVI-6226

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