Published 2014
| Version v1
Publication
Can fluorescence and SHG data be enriched by Müller matrix signature
Description
The most recent advances in optical microscopy are mainly focused towards superresolution,
using fluorescence stochastic/targeted read-out methods [1]. Since the demand is growing for
imaging thick biological specimens as cell aggregates (i.e., tumor spheroids), tissues/organs
(i.e. ligaments, meniscus) and small organisms (i.e. zebrafish), scattering represents a key
aspect in image analysis and reconstruction [2]. Additional information, if exploited
correctly, improves the accuracy of any measurements giving rise, in the specific case of
optical microscopy, to an increase in the bandwidth and hence resolution of the system [3].
So far, polarization properties of the incoming/outcoming light have been shown to be able to
provide further information about the sample [4, 5]. Here the attention is given to the
possibility of utilizing a Mueller matrix analysis of the signal in order to extract information
about optically active biological structures in the sample, with particular interest in chiral
objects [6-8]. Since Mueller matrix analysis is generally used to study polarization properties
in angular scattering, an ongoing question is: can fluorescence and SHG data be enriched by
Mueller matrix signature, too? As the possibility was demonstrated of obtaining
ultrastructural information about chromatin-DNA organization using circular intensity
differential light scattering [9], the Mueller matrix integrated approach could allow progress
towards label free imaging. A possible Mueller matrix polarimetry integrated architecture
will be outlined, based on photoelastic modulation (vs. Pockels cell) as a polarization
generator along the excitation pathway [9-13]. A classical electrodynamics model will be
reported about circular intensity differential scattering of chromatin-DNA in a label-free
perspective [14]. In addition, some preliminary raw data arising from SHG measurements
will be discussed.
Additional details
Identifiers
- URL
- http://hdl.handle.net/11567/813101
- URN
- urn:oai:iris.unige.it:11567/813101