NtcA is responsible for accumulation of the small isoform of ferredoxin:NADP oxidoreductase

Description

In several cyanobacteria, petH, the gene encoding ferredoxin:NADP oxidoreductase (FNR), is transcribed from at least two promoters depending on growth conditions. Two transcripts (short and long) are translated from two different translation initiation sites, resulting in two isoforms (large and small, respectively). Here, we show that in Synechocystis PCC6803 the global transcriptional regulator NtcA activates transcription from the distal petH promoter. Modification of the NtcA-binding site prevents NtcA binding to the promoter in vitro and abolishes accumulation of the small isoform of FNR in vivo. We also demonstrate that a similar petH transcription and translation regime occurs in other cyanobacteria. The conditions under which this system operates provide hints for the function of each FNR isoform.

Abstract

Centre National dela Recherche Scientifique UMR8221

Abstract

Commisariat à l'Energie Atomique Bioénergies DSV IBITEC-S

Abstract

Ministerio deEconomía y Competitividad, FEDER BFU2010-15708

Abstract

Junta de Andalucía P07-CVI-02792 BIO-284

Additional details