Published January 12, 2024
| Version v1
Publication
Chitosan biofilms: Insights for the selective electromembrane extraction of fluoroquinolones from biological samples
Contributors
Others:
- Universidad de Sevilla. Departamento de Química Analítica
- Universidad de Sevilla. Departamento de Química Física
- Universidad de Sevilla. FQM106: Carbolinas
- European Commission (EC). Fondo Europeo de Desarrollo Regional (FEDER)
- Ministerio de Ciencia e Innovación (MICIN). España
- Agencia Estatal de Investigación. España
- Ministerio de Ciencia, Innovación y Universidades (MICINN). España
- Junta de Andalucía
Description
A selective electromembrane extraction procedure for the extraction of Enrofloxacin, Marbofloxacin and
Flumequine, usually employed as antibiotic in veterinarian use, is proposed by using a chitosan biofilm,
composed by 60% (w/w) chitosan and 40% (w/w) Aliquat®336, as active biopolymeric support. The
interaction mechanism occurring between the target drugs and the biopolymer has been deeply studied
using the Quantum Theory of Atoms in Molecules. The obtained results show the interaction between the
extracted fluoroquinolones and the biomembrane is stabilized by two hydrogen bonds formed between
both the carboxyl and keto groups of the drugs with both the amine and hydroxyl groups of glucosamine
in the biopolymer. The energetic results agree with the high extraction efficiency obtained for Marbofloxacin, Enrofloxacin and Flumequine in terms of enrichment factors (83, 82 and 58, respectively) in
presence of other fluoroquinolones. Under optimum conditions, the proposed electromembrane
extraction method exhibits wide linear ranges of 4.2e200 mg L1
, 5.6e200 mg L1 and 5.1e200 mg L1
,
respectively; low limits of detection close to 1.3 mg L1 and appropriate repeatability (relative standard
deviation values 4e7%).
Abstract
Fondos Europeos FEDER, Ministerio de Ciencia e Innovación y Agencia Estatal de Investigación, de España - PGC2018-096608- B-C22Abstract
Ministerio de Ciencia, Innovación y Universidades de España - RED2018-102522-TAbstract
Junta de Andalucía - 2019/FQM-106Additional details
Identifiers
- URL
- https://idus.us.es/handle//11441/153283
- URN
- urn:oai:idus.us.es:11441/153283