Published 2018
| Version v1
Publication
Pre-ART HIV-1 DNA in CD4+ T cells correlates with baseline VIRO-immunological status and outcome in patients under first-line ART
Creators
- Ceccherini-Silberstein F.
- Cozzi Lepri A.
- Alteri C.
- Merlini E.
- Surdo M.
- Marchetti G.
- Capobianchi M. R.
- De Luca A.
- Gianotti N.
- Viale P.
- Andreoni M.
- Antinori A.
- Perno C. F.
- D'Arminio Monforte A.
- Castagna A.
- Castelli F.
- Cauda R.
- Di Perri G.
- Galli M.
- Iardino R.
- Ippolito G.
- Lazzarin A.
- Marchetti G. C.
- Rezza G.
- Von Schloesser F.
- Cozzi-Lepri A.
- Girardi E.
- Lo Caputo S.
- Mussini C.
- Puoti M.
- Ammassari A.
- Balotta C.
- Bandera A.
- Bonfanti P.
- Bonora S.
- Borderi M.
- Calcagno A.
- Calza L.
- Cingolani A.
- Cinque P.
- Di Biagio A.
- Gori A.
- Guaraldi G.
- Lapadula G.
- Lichtner M.
- Madeddu G.
- Maggiolo F.
- Marcotullio S.
- Monno L.
- Nozza S.
- Quiros Roldan E.
- Rossotti R.
- Rusconi S.
- Santoro M. M.
- Saracino A.
- Zaccarelli M.
- Fanti I.
- Galli L.
- Lorenzini P.
- Rodano A.
- Shanyinde M.
- Tavelli A.
- Carletti F.
- Carrara S.
- Di Caro A.
- Graziano S.
- Petrone F.
- Prota G.
- Quartu S.
- Truffa S.
- Giacometti A.
- Costantini A.
- Barocci V.
- Angarano G.
- Santoro C.
- Suardi C.
- Donati V.
- Verucchi G.
- Minardi C.
- Quirino T.
- Abeli C.
- Manconi P. E.
- Piano P.
- Cacopardo B.
- Celesia B.
- Vecchiet J.
- Falasca K.
- Sighinolfi L.
- Segala D.
- Blanc P.
- Vichi F.
- Cassola G.
- Viscoli C.
- Alessandrini A.
- Bobbio N.
- Mazzarello G.
- Mastroianni C.
- Pozzetto I.
- Molteni C.
- Chiodera A.
- Milini P.
- Nunnari G.
- Pellicano G.
- Rizzardini G.
- Moioli M. C.
- Piolini R.
- Ridolfo A. L.
- Salpietro S.
- Tincati C.
- Puzzolante C.
- Chirianni A.
- Borgia G.
- Esposito V.
- Orlando R.
- Bonadies G.
- Di Martino F.
- Gentile I.
- Maddaloni L.
- Cattelan A. M.
- Marinello S.
- Cascio A.
- Colomba C.
- Baldelli F.
- Schiaroli E.
- Parruti G.
- Sozio F.
- Magnani G.
- Ursitti M. A.
- Acinapura R.
- Baldin G.
- Capozzi M.
- Cicalini S.
- Cristaudo A.
- Fontanelli Sulekova L.
- Iaiani G.
- Latini A.
- Mastrorosa I.
- Plazzi M. M.
- Savinelli S.
- Vergori A.
- Vullo V.
- Cecchetto M.
- Viviani F.
- Bagella P.
- Rossetti B.
- Franco A.
- Fontana Del Vecchio R.
- Francisci D.
- Di Giuli C.
- Caramello P.
- Orofino G. C.
- Sciandra M.
- Bassetti M.
- Londero A.
- Pellizzer G.
- Manfrin V.
- Starnini G.
- Ialungo A.
Contributors
Others:
- Ceccherini-Silberstein, F.
- Cozzi Lepri, A.
- Alteri, C.
- Merlini, E.
- Surdo, M.
- Marchetti, G.
- Capobianchi, M. R.
- De Luca, A.
- Gianotti, N.
- Viale, P.
- Andreoni, M.
- Antinori, A.
- Perno, C. F.
- D'Arminio Monforte, A.
- Castagna, A.
- Castelli, F.
- Cauda, R.
- Di Perri, G.
- Galli, M.
- Iardino, R.
- Ippolito, G.
- Lazzarin, A.
- Marchetti, G. C.
- Rezza, G.
- Von Schloesser, F.
- Cozzi-Lepri, A.
- Girardi, E.
- Lo Caputo, S.
- Mussini, C.
- Puoti, M.
- Ammassari, A.
- Balotta, C.
- Bandera, A.
- Bonfanti, P.
- Bonora, S.
- Borderi, M.
- Calcagno, A.
- Calza, L.
- Cingolani, A.
- Cinque, P.
- Di Biagio, A.
- Gori, A.
- Guaraldi, G.
- Lapadula, G.
- Lichtner, M.
- Madeddu, G.
- Maggiolo, F.
- Marcotullio, S.
- Monno, L.
- Nozza, S.
- Quiros Roldan, E.
- Rossotti, R.
- Rusconi, S.
- Santoro, M. M.
- Saracino, A.
- Zaccarelli, M.
- Fanti, I.
- Galli, L.
- Lorenzini, P.
- Rodano, A.
- Shanyinde, M.
- Tavelli, A.
- Carletti, F.
- Carrara, S.
- Di Caro, A.
- Graziano, S.
- Petrone, F.
- Prota, G.
- Quartu, S.
- Truffa, S.
- Giacometti, A.
- Costantini, A.
- Barocci, V.
- Angarano, G.
- Santoro, C.
- Suardi, C.
- Donati, V.
- Verucchi, G.
- Minardi, C.
- Quirino, T.
- Abeli, C.
- Manconi, P. E.
- Piano, P.
- Cacopardo, B.
- Celesia, B.
- Vecchiet, J.
- Falasca, K.
- Sighinolfi, L.
- Segala, D.
- Blanc, P.
- Vichi, F.
- Cassola, G.
- Viscoli, C.
- Alessandrini, A.
- Bobbio, N.
- Mazzarello, G.
- Mastroianni, C.
- Pozzetto, I.
- Molteni, C.
- Chiodera, A.
- Milini, P.
- Nunnari, G.
- Pellicano, G.
- Rizzardini, G.
- Moioli, M. C.
- Piolini, R.
- Ridolfo, A. L.
- Salpietro, S.
- Tincati, C.
- Puzzolante, C.
- Chirianni, A.
- Borgia, G.
- Esposito, V.
- Orlando, R.
- Bonadies, G.
- Di Martino, F.
- Gentile, I.
- Maddaloni, L.
- Cattelan, A. M.
- Marinello, S.
- Cascio, A.
- Colomba, C.
- Baldelli, F.
- Schiaroli, E.
- Parruti, G.
- Sozio, F.
- Magnani, G.
- Ursitti, M. A.
- Acinapura, R.
- Baldin, G.
- Capozzi, M.
- Cicalini, S.
- Cristaudo, A.
- Fontanelli Sulekova, L.
- Iaiani, G.
- Latini, A.
- Mastrorosa, I.
- Plazzi, M. M.
- Savinelli, S.
- Vergori, A.
- Vullo, V.
- Cecchetto, M.
- Viviani, F.
- Bagella, P.
- Rossetti, B.
- Franco, A.
- Fontana Del Vecchio, R.
- Francisci, D.
- Di Giuli, C.
- Caramello, P.
- Orofino, G. C.
- Sciandra, M.
- Bassetti, M.
- Londero, A.
- Pellizzer, G.
- Manfrin, V.
- Starnini, G.
- Ialungo, A.
Description
Objectives We evaluated the association between pre-ART HIV DNA and HIV-infected participant characteristics at baseline as well as with their response to first-line ART. Methods Four hundred and thirty-three patients from the ICONA cohort, starting first-line ART after the year 2000, were analysed. Pre-ART HIV DNA was quantified with the modified COBAS TaqMan HIV-1 Test and normalized by CD4+ T cells. Linear correlation between pre-ART HIV DNA and other continuous markers (HIV RNA, CD4 count, markers of inflammation and coagulation) at baseline was evaluated by means of Pearson correlation coefficient and a linear regression model. Survival analyses and Cox regression models were used to study the association between pre-ART HIV DNA and time to VIRO-immunoclinical events. Results Pre-ART HIV DNA [median (IQR): 10 € 702 (3397-36 € 632) copies/10 6 CD4+ T cells] was correlated with pre-ART HIV RNA [R 2 = +0.44, (P < 0.0001)], CD4+ T cells [R 2 = '0.58, (P < 0.0001)] and CD4/CD8 ratio [R 2 = '0.48, (P < 0.0001)], while weaker correlations were observed with CD8+ T cells (R 2 = '0.20, P = 0.01), IL-6 (R 2 = +0.16, P = 0.002) and soluble CD14 (R 2 = +0.09, P = 0.05). Patients with higher pre-ART HIV DNA showed lower rate and delayed VIROlogical response (defined as HIV RNA ≤50 copies/mL), compared with those having lower HIV DNA (67.2% for >10 € 000, 81.1% for 1000-10 € 000 and 86.4% for 10-1000 copies/10 6 CD4+ T cells; P = 0.0004). Higher pre-ART HIV DNA was also correlated with increased risk of VIROlogical rebound (defined as HIV RNA >50 copies/mL) by 24 months (17.2% for >10 € 000, 7.4% for 1000-10 € 000 and 4.3% for 10-1000 copies/10 6 CD4+ T cells; P = 0.0048). Adjusted HRs of all VIROlogical rebound definitions confirmed these findings (P ≤ 0.02). Conclusions Pre-ART HIV DNA, along with HIV RNA and CD4+ T cell count, should be considered as a new staging marker to better identify people at lower (or higher) risk of viral rebound following achievement of VIROlogical suppression (≤50 copies/mL).
Additional details
Identifiers
- URL
- http://hdl.handle.net/11567/950179
- URN
- urn:oai:iris.unige.it:11567/950179
Origin repository
- Origin repository
- UNIGE