Published 2018
| Version v1
Journal article
Intercalibration of counting methods for Ostreopsis spp. blooms in the Mediterranean Sea
Creators
- Vassalli, Massimo
- Penna, Antonella
- Sbrana, Francesca
- Casabianca, Silvia
- Gjeci, Nikolla
- Capellacci, Samuela
- Asnaghi, Valentina
- Ottaviani, Ennio
- Giussani, Valentina
- Pugliese, Laura
- Jauzein, Cecile
- Lemée, Rodolphe
- Hachani, Mohamed Amine
- Turki, Souad
- Acaf, Laury
- Saab, Marie Abboud-Abi
- Fricke, Anna
- Mangialajo, Luisa
- Bertolotto, Rosella
- Totti, Cecilia
- Accoroni, Stefano
- Berdalet, Elisa
- Vila, Magda
- Chiantore, Mariachiara
Contributors
Others:
- James Watt School of Engineering [Univ Glasgow] ; University of Glasgow
- Università di Urbino ; Università di Urbino
- Department of Earth, Environmental and Life Sciences (DISTAV) ; Università degli studi di Genova = University of Genoa (UniGe)
- OnAIR srl ; OnAIR srl
- Laboratoire d'océanographie de Villefranche (LOV) ; Observatoire océanologique de Villefranche-sur-mer (OOVM) ; Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)
- Institut National des Sciences et Technologies de la Mer ; Centre La Goulette
- Laboratoire Chrono-environnement - CNRS - UBFC (UMR 6249) (LCE) ; Centre National de la Recherche Scientifique (CNRS)-Université de Franche-Comté (UFC) ; Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC)
- Istituto Nazionale di Geofisica e di Oceanografia Sperimentale (OGS)
- Laboratoire d'océanographie de Villefranche (LOV) ; Institut national des sciences de l'Univers (INSU - CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Institut de la Mer de Villefranche (IMEV) ; Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)
- Ecosystèmes Côtiers Marins et Réponses aux Stress (ECOMERS) ; Université Nice Sophia Antipolis (1965 - 2019) (UNS) ; COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Université Côte d'Azur (UCA)
- Consejo Superior de Investigaciones Científicas [Madrid] (CSIC)
Description
This paper describes the adoption and validation of two innovative methods for the automated count of Ostreopsis spp. concentration in sea water: a molecular assay based on RT-qPCR and an opto-electronic device implementing automatic recognition algorithms. The proposed approaches were tested on samples coming from different locations along the Mediterranean Sea and compared with the standard counting method based on microscopy observation by a taxonomy expert. The results demonstrate the effectiveness of both automatic approaches which provide a valuable tool, mostly cost and time effective, for the establishment of wide pan Mediterranean monitoring strategies of Ostreopsis spp. blooms. Moreover, the two automatic methods demonstrated the ability to discriminate for the presence of a different but similar species, O. fattorussoi, for which new species-specific qPCR primers were developed.
Additional details
Identifiers
- URL
- https://hal.archives-ouvertes.fr/hal-03505068
- URN
- urn:oai:HAL:hal-03505068v1