Quantitative analysis of protein-RNA interactions in fission yeast

Description

Characterizing the interactions between RNAs and proteins in vivo is key to better understand how organisms regulate gene expression. Here, we describe a robust and quantitative protocol to measure specific RNA-protein interactions in a native context using RNA immunoprecipitation (RIP). We provide a comprehensive experimental framework to detect cotranslational interactions and detail the quantitative analysis of purified RNAs by PCR and high-throughput sequencing. Although we developed the protocol in fission yeast, it can be readily implemented in other yeast species. For complete details on the use and execution of this protocol, please refer to Toullec et al. (2021).

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Fondation pour la Recherche Médicale SPF20130526854

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Biotechnology and Biological Sciences Research Council (BBSRC) BB/G011869/1, BB/S015833/1

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Agence Nationale de la Recherche ANR-15-CE12-0009-01, ANR-15-CE11-0022-03

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Fondation ARC pour la recherche sur le cancer PJA 20181208277

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