Published July 23, 2019
| Version v1
Journal article
In Vivo Assessment of VCAM-1 Expression by SPECT/CT Imaging in Mice Models of Human Triple Negative Breast Cancer
Creators
- Montemagno, Christopher
- Dumas, Laurent
- Cavaillès, Pierre
- Ahmadi, Mitra
- Bacot, Sandrine
- Debiossat, Marlène
- Soubies, Audrey
- Djaïleb, Loic
- Leenhardt, Julien
- de Leiris, Nicolas
- Dufies, Maeva
- Pagès, Gilles
- Hernot, Sophie
- Devoogdt, Nick
- Perret, Pascale
- Riou, Laurent
- Fagret, Daniel
- Ghezzi, Catherine
- Broisat, Alexis
Contributors
Others:
- Radiopharmaceutiques biocliniques (LRB) ; Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Grenoble Alpes (UGA)
- Institut de Recherche sur le Cancer et le Vieillissement (IRCAN) ; Université Nice Sophia Antipolis (1965 - 2019) (UNS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Côte d'Azur (UniCA)
Description
Recent progress in breast cancer research has led to the identification of Vascular Cell Adhesion Molecule-1 (VCAM-1) as a key actor of metastatic colonization. VCAM-1 promotes lung-metastases and is associated with clinical early recurrence and poor outcome in triple negative breast cancer (TNBC). Our objective was to perform the in vivo imaging of VCAM-1 in mice models of TNBC. The Cancer Genomic Atlas (TCGA) database was analyzed to evaluate the prognostic role of VCAM-1 in TNBC. MDA-MB-231 (VCAM-1+) and control HCC70 (VCAM-1-) TNBC cells were subcutaneously xenografted in mice and VCAM-1 expression was assessed in vivo by single-photon emission computed tomography (SPECT) imaging using 99mTc-cAbVCAM1-5. Then, MDA-MB-231 cells were intravenously injected in mice and VCAM-1 expression in lung metastasis was assessed by SPECT imaging after 8 weeks. TCGA analysis showed that VCAM-1 is associated with a poor prognosis in TNBC patients. In subcutaneous tumor models, 99mTc-cAbVCAM1-5 uptake was 2-fold higher in MDA-MB-231 than in HCC70 (p < 0.01), and 4-fold higher than that of the irrelevant control (p < 0.01). Moreover, 99mTc-cAbVCAM1-5 uptake in MDA-MB-231 lung metastases was also higher than that of 99mTc-Ctl (p < 0.05). 99mTc-cAbVCAM1-5 is therefore a suitable tool to evaluate the role of VCAM-1 as a marker of tumor aggressiveness of TNBC.
Abstract
International audienceAdditional details
Identifiers
- URL
- https://hal.science/hal-04767695
- URN
- urn:oai:HAL:hal-04767695v1
Origin repository
- Origin repository
- UNICA