p38MAP Kinase activity is required for human primary adipocyte differentiation.

Creators: Aouadi, Myriam; Jager, Jennifer; Laurent, Kathiane; Gonzalez, Teresa; Cormont, Mireille; Binétruy, Bernard; Le Marchand Brustel, Yannick; Tanti, Jean-François; Bost, Frédéric

Others:: Signalisation moléculaire et obésité ; Université Nice Sophia Antipolis (1965 - 2019) (UNS) ; COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-IFR50-Institut National de la Santé et de la Recherche Médicale (INSERM); Fibrinolyse et Pathologie Vasculaire ; Université de la Méditerranée - Aix-Marseille 2-Institut National de la Santé et de la Recherche Médicale (INSERM); CHU Nice ; Centre Hospitalier Universitaire de Nice (CHU Nice)

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Little is known about the role of p38MAPK in human adipocyte differentiation. Here we showed that p38MAPK activity increases during human preadipocytes differentiation. Pharmacological inhibition of p38MAPK during adipocyte differentiation of primary human preadipocytes markedly reduced triglycerides accumulation and adipocyte markers expression. Cell cycle arrest or proliferation was not affected by p38MAPK inhibition. Although induction of C/EBPbeta was not altered by the p38MAPK inhibitor, its phosphorylation on Threonine(188) was decreased as well as PPARgamma expression. These results indicate that p38MAPK plays a positive role in human adipogenesis through regulation of C/EBPbeta and PPARgamma factors.

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p38MAP Kinase activity is required for human primary adipocyte differentiation.

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Abstract

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