Published 2017
| Version v1
Journal article
Ezrin enhances line tension along transcellular tunnel edges via NMIIa driven actomyosin cable formation
Creators
Contributors
Others:
- Centre méditerranéen de médecine moléculaire (C3M) ; Université Nice Sophia Antipolis (1965 - 2019) (UNS) ; COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Côte d'Azur (UCA)
- Université Nice Sophia Antipolis - Faculté de Médecine (UNS UFR Médecine) ; Université Nice Sophia Antipolis (1965 - 2019) (UNS) ; COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)
- Laboratoire de Chimie et Physique - Approche Multi-échelle des Milieux Complexes (LCP-A2MC) ; Université de Lorraine (UL)
- Helsingin yliopisto = Helsingfors universitet = University of Helsinki
- University of Pennsylvania [Philadelphia]
- Centre d'Infection et d'Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 (CIIL) ; Institut Pasteur de Lille ; Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille)-Centre National de la Recherche Scientifique (CNRS)
- Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille)
- Sorbonne Université (SU)
- Laboratoire Physico-Chimie Curie [Institut Curie] (PCC) ; Institut Curie [Paris]-Institut de Chimie du CNRS (INC)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)
Description
Transendothelial cell macroaperture (TEM) tunnels control endothelium barrier function and are triggered by several toxins from pathogenic bacteria that provoke vascular leakage. Cellular dewetting theory predicted that a line tension of uncharacterized origin works at TEM boundaries to limit their widening. Here, by conducting high-resolution microscopy approaches we unveil the presence of an actomyosin cable encircling TEMs. We develop a theoretical cellular dewetting framework to interpret TEM physical parameters that are quantitatively determined by laser ablation experiments. This establishes the critical role of ezrin and non-muscle myosin II (NMII) in the progressive implementation of line tension. Mechanistically, fluorescence-recovery-after-photobleaching experiments point for the upstream role of ezrin in stabilizing actin filaments at the edges of TEMs, thereby favouring their crosslinking by NMIIa. Collectively, our findings ascribe to ezrin and NMIIa a critical function of enhancing line tension at the cell boundary surrounding the TEMs by promoting the formation of an actomyosin ring.
Abstract
International audienceAdditional details
Identifiers
- URL
- https://hal.sorbonne-universite.fr/hal-01960028
- URN
- urn:oai:HAL:hal-01960028v1
Origin repository
- Origin repository
- UNICA