Published 2005
| Version v1
Publication
ALP enzymatic activity related to different implant surface microtopographies
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Contributors
Description
The aim of this study was an in vitro investigation of the Alkaline
Phosphatase (ALP) enzymatic activity related to different surface
treatments applied to ten implant systems in order to assess if the
interaction between cells and implant (osteoblastic proliferation and
differentiation) was influenced by the surface structure and/or
surface composition of the fixture. The originality of this study
was that all implants were tested as manufactured for clinical use.
The implant systems object of this study divided by the surface
treatment were the following: Machined: Mk III Branemark;
Sandblasted: Ankylos, Silhouette and Galant (experimental fixture);
Etched: Osseotite, Mac System,MK 4 (experimental fixture),
ITI; Oxided: Ti Unite, Pilot. We used Sa-OS2 cultured osteoblasts
and we analyzed n14 fixtures for each implant system (n11 fixture
for the cellular growth curve and n13 fixtures for the ALP activity).
After 14 days the assay for the ALP activity was carried out
according to Wataha et al. (JBMR, 1997) After the cellular growth
evaluation in a Burker's hemocytometer chamber we quantify by a
spectrophotometer at 405nm the absorbance value for each sample
(the absorbance value indicate the amount of the conversion by ALP
of the P-Nitro-Phenyl-Phosphate into P-Nitro-Phenol) and we
corrected the mean value for cell number determined before. The
data were statistically analyzed by ANOVA and Post hoc Scheffe`
Test. Within the limits of the in vitro investigations we can
conclude that:
The etched surfaces shown more cellular growth than others.
The sandblasted surfaces shown the smallest amount of
cellular proliferation but a very high differentiation (according
to Postiglione et al 2003 that found an inverse correlation
between the two factors)
We found a statistically significant difference in ALP activity
only between oxided and etched surfaces (p < 0.05)
The sandblasted and oxided surfaces shown more osteoblastic
differentiation (more ALP enzymatic activity)
Themachined surface is competitive, as regard the osteoblastic
differentiation, with the rougher surfaces.
Actually it's still difficult to recommend a particular rougher
surface.
Additional details
Identifiers
- URL
- http://hdl.handle.net/11567/202830
- URN
- urn:oai:iris.unige.it:11567/202830