Published September 6, 2021
| Version v1
Journal article
Brown adipose tissue monocytes support tissue expansion
Creators
- Gallerand, Alexandre
- Stunault, Marion
- Merlin, Johanna
- Luehmann, Hannah
- Sultan, Deborah
- Firulyova, Maria
- Magnone, Virginie
- Khedher, Narges
- Jalil, Antoine
- Dolfi, Bastien
- Castiglione, Alexia
- Dumont, Adelie
- Ayrault, Marion
- Vaillant, Nathalie
- Gilleron, Jérôme
- Barbry, Pascal
- Dombrowicz, David
- Mack, Matthias
- Masson, David
- Bertero, Thomas
- Becher, Burkhard
- Williams, Jesse
- Zaitsev, Konstantin
- Liu, Yongjian
- Guinamard, Rodolphe r.
- Yvan-Charvet, Laurent
- Ivanov, Stoyan
Contributors
Others:
- Centre méditerranéen de médecine moléculaire (C3M) ; Université Nice Sophia Antipolis (1965 - 2019) (UNS) ; COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Côte d'Azur (UCA)
- Washington University in Saint Louis (WUSTL)
- ITMO University [Russia]
- Institut de pharmacologie moléculaire et cellulaire (IPMC) ; Université Nice Sophia Antipolis (1965 - 2019) (UNS) ; COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Centre National de la Recherche Scientifique (CNRS)-Université Côte d'Azur (UCA)
- Lipides - Nutrition - Cancer [Dijon - U1231] (LNC) ; Université de Bourgogne (UB)-Institut National de la Santé et de la Recherche Médicale (INSERM)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement
- Récepteurs Nucléaires, Maladies Métaboliques et Cardiovasculaires (RNMCD - U1011) ; Institut Pasteur de Lille ; Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille)
- University Hospital Regensburg
- Institute of Experimental Immunology [Zurich]
- University of Minnesota Medical School ; University of Minnesota System
- Washington University School of Medicine in St. Louis ; Washington University in Saint Louis (WUSTL)
- Centre National de la Recherche Scientifique (CNRS)
Description
Monocytes are part of the mononuclear phagocytic system. Monocytes play a central role during inflammatory conditions and a better understanding of their dynamics might open therapeutic opportunities. In the present study, we focused on the characterization and impact of monocytes on brown adipose tissue (BAT) functions during tissue remodeling. Single-cell RNA sequencing analysis of BAT immune cells uncovered a large diversity in monocyte and macrophage populations. Fate-mapping experiments demonstrated that the BAT macrophage pool requires constant replenishment from monocytes. Using a genetic model of BAT expansion, we found that brown fat monocyte numbers were selectively increased in this scenario. This observation was confirmed using a CCR2-binding radiotracer and positron emission tomography. Importantly, in line with their tissue recruitment, blood monocyte counts were decreased while bone marrow hematopoiesis was not affected. Monocyte depletion prevented brown adipose tissue expansion and altered its architecture. Podoplanin engagement is strictly required for BAT expansion. Together, these data redefine the diversity of immune cells in the BAT and emphasize the role of monocyte recruitment for tissue remodeling.
Abstract
International audienceAdditional details
Identifiers
- URL
- https://hal.archives-ouvertes.fr/hal-03473176
- URN
- urn:oai:HAL:hal-03473176v1
Origin repository
- Origin repository
- UNICA