FLIM-FRET of Chromatin in Live Cells using Two DNA-Binding Dyes

Description

The structural state of DNA in mammalian nucleus of living cells corresponds to varying levels of chromatin organization, ranging from the nucleosomes to higher order structures. This varying degree of hierarchical compaction is integral to genome functions, allowing or preventing access to several genetic nuclear factors (epigenetic, replicative, and transcriptional)[Misteli T, Cell (2007)]. However, how the higher-order chromatin structures are formed and then behave in various cellular processes in live cells remains unclear.

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