Published 2007
| Version v1
Journal article
Osmotic Regulation of Cellular Glucose Uptake
Contributors
Others:
- Centre méditerranéen de médecine moléculaire (C3M) ; Université Nice Sophia Antipolis (1965 - 2019) (UNS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Côte d'Azur (UniCA)
- Nutrition, obésité et risque thrombotique (NORT) ; Institut National de la Recherche Agronomique (INRA)-Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)
- Signalisation moléculaire et obésité ; Université Nice Sophia Antipolis (1965 - 2019) (UNS)-IFR50-Institut National de la Santé et de la Recherche Médicale (INSERM)
Description
This chapter describes various approaches allowing the study of hyperosmolarity in the functions of 3T3-L1 adipocytes. Hyperosmolarity mimics insulin responses, such as glucose uptake and membrane ruffling, but also antagonizes these insulin effects, which can be evaluated in 3T3-L1 adipocytes. The molecular mechanisms of these effects can be also investigated by measuring the activation of different signaling pathways: (i) the phosphorylation of docking proteins on tyrosine and serine residues (serines 307 and 632), (ii) the phosphorylation of serine/threonine kinases, and (iii) the activation of phosphatidylinositol 3-kinase.
Abstract
International audienceAdditional details
Identifiers
- URL
- https://hal.science/hal-04496799
- URN
- urn:oai:HAL:hal-04496799v1
Origin repository
- Origin repository
- UNICA