Anti-obesogenic effects of vegetable-derived protein hydrolysate [Dataset]

Description

Data was acquired for the study published in the article titled: Anti-obesogenic effect of lupin-derived protein hydrolysate through modulation of adiposopathy, insulin resistance and gut dysbiosis in a diet-induced obese mouse. This study examines the effects of a lupin protein hydrolysate (LPH) on obesity, adipose tissue dysfunction and gut dysbiosis in obese mice. LPH was characterized and protein concentration, ashes, moisture, fiber, fats, soluble sugars and phenol content were measured as described. Amino acid composition was determined using standard amino acid mix solution. The molecular weight profile was obtained by molecular exclusion chromatography. Eight-week-old male C57BL6/N mice were housed under standard conditions and mice were randomly assigned to three groups: mice fed a standard diet (SD), a high-fat diet (HFD) or an HFD and treated intragastrically with LPH (HFD+LPH) at a dose of 100mg/kg five days a week for 12 weeks. The SD and HFD groups were treated with vehicle under the same conditions as the HFD+LPH group. Food consumption and individual body weight were measured weekly. Animals were sacrificed with an intraperitoneal injection of sodium thiopental and blood was collected by cardiac puncture. Epidydimal adipose tissue (EpiWAT) was dissected, snap-frozen and stored at -80ºC until use or fixed in a 4% paraformaldehyde (PFA) solution. Stool was collected prior to euthanasia and stored at -80ºC. Serum biochemical profile was measured by chemoluminiscence in the Cobas Integra 400 (Roche Diagnostics, Indianapolis, IN, USA) at the Estación Biológica de Doñana (EBD-CSIC, Seville, Spain). Serum concentrations of leptin, adiponectin and insulin were quantified using enzyme-linked immunosorbent assays (ELISA) kits. Adipose tissue samples fixed in 4% PFA were dehydrated, embedded in paraffin blocks and sliced 4μm thick, stained with hematoxylin-eosin and cover mounted for posterior observation. Photomicrographs were obtained using Leica THUNDER microscope and Leica Application Suite X software. Adipocyte morphology and number of crown-like structures was blinded analyzed using ImageJ v1.53h public software. High throughput 16S rRNA gene amplicon analysis was performed using total DNA extracted from stool. 16S rRNA V3–V4 region was sequenced using MiSeq protocol and instruments. The raw sequences were processed using mothur.

Abstract

Dataset entitled "hydrolysate_composition.xlsx" includes macromolecular and aminoacidic composition (first sheet) and chromatogram information (second sheet) of LPH obtained from three independent measurements. Dataset entitled "obesity_markers.xlsx" includes experimental group and numerical data (columns) obtained from the measurement of parameters from animals (rows) used in the present study.

Abstract

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