Development of silica‐based biodegradable submicrometric carriers and investigating their characteristics as in vitro delivery vehicles

Description

Nanostructured silica (SiO2)-based materials are attractive carriers for the delivery of bioactive compounds into cells. In this study, we developed hollow submicrometric particles composed of SiO2 capsules that were separately loaded with various bioactive molecules such as dextran, proteins, and nucleic acids. The structural characterization of the reported carriers was conducted using transmission and scanning electron microscopies (TEM/SEM), confocal laser scanning microscopy (CLSM), and dynamic light scattering (DLS). Moreover, the interaction of the developed carriers with cell lines was studied using standard viability, proliferation, and uptake assays. The submicrometric SiO2-based capsules loaded with DNA plasmid encoding green fluorescence proteins (GFP) were used to transfect cell lines. The obtained results were compared with studies made with similar capsules composed of polymers and show that SiO2-based capsules provide better transfection rates on the costs of higher toxicity.

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Deutsche Forschungsgemeinschaft-DFG A 794/21-1

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VI Plan de Investigación y Transferencia Tecnológica de la Universidad de Sevilla-VI PPIT-US

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Instituto de Investigacones Químicas (IIQ-CSIC), Universidad de Sevilla y Ministerio de Economía y Competitividad de España-CTQ2016-78580-C2-1-R

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European Cooperation in Science and Technology de la Unión Europea-COST CA-18132

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Russian Foundation for Basic Research-RFBR 19-015-00098

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