Published 2019 | Version v1
Publication Metadata-only

Human CAR NK Cells: A New Non-viral Method Allowing High Efficient Transfection and Strong Tumor Cell Killing

Ingegnere T.
Mariotti F. R.
Pelosi A.
Quintarelli C.
De Angelis B.
Tumino N.
Besi F.
Cantoni C.
Locatelli F.
Vacca P.
Moretta L.
Others:
Ingegnere, T.
Mariotti, F. R.
Pelosi, A.
Quintarelli, C.
De Angelis, B.
Tumino, N.
Besi, F.
Cantoni, C.
Locatelli, F.
Vacca, P.
Moretta, L.

Description

CAR-NK cells may represent a valuable tool, complementary to CAR-T cells, in adoptive immunotherapy of leukemia and solid tumors. However, gene transfer to human NK cells is a challenging task, particularly with non-virus-based techniques. Here, we describe a new procedure allowing efficient electroporation-based transfection of plasmid DNA, including CAR and CCR7 genes, in resting or cytokine-expanded human NK cell populations and NK-92 cell line. This procedure may offer a suitable platform for a safe and effective use of CAR-NK cells in adoptive immunotherapy of cancer.

Additional details

Identifiers Origin repository
Created:
April 14, 2023
Modified:
November 27, 2023