Nuclear/Cytoplasmic Fractionation of Proteins from Caenorhabditis elegans

Description

C. elegans is widely used to investigate biological processes related to health and disease. To study protein localization, fluorescently-tagged proteins can be used in vivo or immunohistochemistry can be performed in whole worms. Here, we describe a technique to localize a protein of interest at a subcellular level in C. elegans lysates, which can give insight into the location, function and/or toxicity of proteins

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National Institutes of Health National Centre for Research Resources (NIH)

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European Research Council (ERC)

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USANIH National Center for Research Resources (NCRR)

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Japan National BioResource Project

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