Published January 24, 2024 | Version v1
Publication

Quantitative Real-Time PCR for Detection of Acinetobacter baumannii Colonization in the Hospital Environment

Description

A real-time PCR assay was developed for detecting the presence of Acinetobacter baumannii on hospital equipment and compared to conventional bacterial culture using 100 hospital environmental samples. The real-time PCR detected contaminated surfaces in 4 h with high sensitivity (100%) compared to conventional culture. Thirty-eight percent of samples were positive by real-time PCR and negative by bacterial culture (false positives), possibly indicating the widespread presence of bacterial DNA that is not associated with viable bacteria.

Additional details

Created:
January 26, 2024
Modified:
January 26, 2024