Published September 10, 2014
| Version v1
Journal article
Screening in Planarians Identifies MORN2 as a Key Component in LC3-Associated Phagocytosis and Resistance to Bacterial Infection
Creators
- Abnave, Prasad
- Mottola, Giovanna
- Gimenez, Gregory
- Boucherit, Nicolas
- Trouplin, Virginie
- Torre, Cedric
- Conti, Filippo
- Ben Amara, Amira
- Lépolard, Catherine
- Djian, Benjamin
- Hamaoui, Daniel
- Mettouchi, Amel
- Kumar, Atul
- Pagnotta, Sophie
- Bonatti, Stefano
- Lepidi, Hubert
- Salvetti, Alessandra
- Abi-Rached, Laurent
- Lemichez, Emmanuel
- Mege, Jean-Louis
- Ghigo, Eric
Contributors
Others:
- Centre méditerranéen de médecine moléculaire (C3M) ; Université Nice Sophia Antipolis (1965 - 2019) (UNS) ; COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Côte d'Azur (UCA)
- Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes (URMITE) ; Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR48 ; Institut des sciences biologiques (INSB-CNRS)-Institut des sciences biologiques (INSB-CNRS)-Centre National de la Recherche Scientifique (CNRS)
- University of Otago [Dunedin, Nouvelle-Zélande]
- Centre Commun de Microscopie Appliquée (CCMA) ; Université Nice Sophia Antipolis (1965 - 2019) (UNS) ; COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)
- Université de Naples
- University of Pisa - Università di Pisa
- Laboratoire d'Analyse, Topologie, Probabilités (LATP) ; Aix Marseille Université (AMU)-École Centrale de Marseille (ECM)-Centre National de la Recherche Scientifique (CNRS)
Description
Dugesia japonica planarian flatworms are naturally exposed to various microbes but typically survive this challenge. We show that planarians eliminate bacteria pathogenic to Homo sapiens, Caenorhabditis elegans, and/or Drosophila melanogaster and thus represent a model to identify innate resistance mechanisms. Whole-transcriptome analysis coupled with RNAi screening of worms infected with Staphylococcus aureus or Legionella pneumophila identified 18 resistance genes with nine human orthologs, of which we examined the function of MORN2. Human MORN2 facilitates phagocytosis-mediated restriction of Mycobacterium tuberculosis, L. pneumophila, and S. aureus in macrophages. MORN2 promotes the recruitment of LC3, an autophagy protein also involved in phagocytosis, to M. tuberculosis-containing phagosomes and subsequent maturation to degradative phagolysosomes. MORN2-driven trafficking of M. tuberculosis to single-membrane, LC3-positive compartments requires autophagy-related proteins Atg5 and Beclin-1, but not Ulk-1 and Atg13, highlighting the importance of MORN2 in LC3-associated phagocytosis. These findings underscore the value of studying planarian defenses to identify immune factors.
Abstract
International audienceAdditional details
Identifiers
- URL
- https://hal.archives-ouvertes.fr/hal-02111891
- URN
- urn:oai:HAL:hal-02111891v1
Origin repository
- Origin repository
- UNICA