TFMLAB: A MATLAB toolbox for 4D traction force microscopy
- Others:
- Universidad de Sevilla. Departamento de Mecánica de Medios Continuos y Teoría de Estructuras
- Cambridge Conservation Initiative (CCI)
- Ministerio de Educación, Cultura y Deporte de España
- Ministerio de Economía y Competitividad de España (MINECO)
- Consejo Europeo de Investigación
- Fonds Wetenschappelijk Onderzoek (FWO)
- KU Leuven
- Hercules
Description
We present TFMLAB, a MATLAB software package for 4D (x;y;z;t) Traction Force Microscopy (TFM). While various TFM computational workflows are available in the literature, open-source programs that are easy to use by researchers with limited technical experience and that can analyze 4D in vitro systems do not exist. TFMLAB integrates all the computational steps to compute active cellular forces from confocal microscopy images, including image processing, cell segmentation, image alignment, matrix displacement measurement and force recovery. Moreover, TFMLAB eases usability by means of interactive graphical user interfaces. This work describes the package's functionalities and analyzes its performance on a real TFM case
Abstract
Article number 100723
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KU Leuven 1S68818N
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Fonds Wetenschappelijk Onderzoek (FWO) V413019N
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Fonds Wetenschappelijk Onderzoek (FWO) G085018N
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Cambridge Conservation Initiative (CCI) 2014TC16RFCB046
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Ministerio de Educación, Cultura y Deporte de España CAS17/00096
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Ministerio de Economía y Competitividad de España (MINECO) PGC2018-097257-B-C31
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Consejo Europeo de Investigación 308223
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KU Leuven C14/17/111
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Fonds Wetenschappelijk Onderzoek (FWO)/Hercules G0H6316N
Additional details
- URL
- https://idus.us.es/handle//11441/137436
- URN
- urn:oai:idus.us.es:11441/137436
- Origin repository
- USE