Published 2018 | Version v1
Journal article Metadata-only

Quantification of salicylic acid (SA) and SA-glucosides in Arabidopsis thaliana

Allasia, Valérie
Industri, Benoit
Ponchet, Michel
Quentin, Michaël
Favery, Bruno
Keller, Harald
Others:
Institut Sophia Agrobiotech (ISA) ; Institut National de la Recherche Agronomique (INRA)-Université Nice Sophia Antipolis (1965 - 2019) (UNS) ; COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Centre National de la Recherche Scientifique (CNRS)
French Government (National Research Agency, ANR) through the 'Investments for the Future' LABEX SIGNALIFE [program reference #ANR-11-LABX0028-01]

Description

Homeostasis between the cytoplasmic plant hormone salicylic acid (SA) and its' inactive,vacuolar storage forms, SA-2-O-β-D-glucoside (SAG) and SA-β-D-Glucose Ester (SGE), regulates thefine-tuning of defense responses to biotrophic pathogens in Arabidopsis thaliana. This protocoldescribes a simplified, optimized procedure to extract and quantify free SA and total hydrolyzable SA inplant tissues using a classical HPLC-based method.

Additional details

Identifiers Origin repository
Created:
December 4, 2022
Modified:
November 29, 2023