Published March 31, 2020
| Version v1
Publication
Sumoylation of Smc5 Promotes Error-free Bypass at Damaged Replication Forks
Description
Replication of a damaged DNA template can threaten the integrity of the genome, requiring the use of various mechanisms to tolerate DNA lesions. The Smc5/6 complex, together with the Nse2/Mms21 SUMO ligase, plays essential roles in genome stability through undefined tasks at damaged replication forks. Various subunits within the Smc5/6 complex are substrates of Nse2, but we currently do not know the role of these modifications. Here we show that sumoylation of Smc5 is targeted to its coiled-coil domain, is upregulated by replication fork damage, and participates in bypass of DNA lesions. smc5-KR mutant cells display defects in formation of sister chromatid junctions and higher translesion synthesis. Also, we provide evidence indicating that Smc5 sumoylation modulates Mph1-dependent fork regression, acting synergistically with other pathways to promote chromosome disjunction. We propose that sumoylation of Smc5 enhances physical remodeling of damaged forks, avoiding the use of a more mutagenic tolerance pathway.
Abstract
Ministerio de Ciencia, Innovacion y Universidades (BFU2015-71308-P, PGC2018-097796-B-I00)Abstract
AGAUR-Generalitat de Catalunya (2017-SGR-569)Additional details
Identifiers
- URL
- https://idus.us.es/handle//11441/94709
- URN
- urn:oai:idus.us.es:11441/94709
Origin repository
- Origin repository
- USE