Tudor staphylococcal nuclease is a docking platform for stress granule components and is essential for SnRK1 activation in Arabidopsis
Citation
Description
Tudor staphylococcal nuclease (TSN; also known as Tudor-SN, p100, or SND1) is a multifunctional, evolutionarily conserved regulator of gene expression, exhibiting cytoprotective activity in animals and plants and oncogenic activity in mammals. During stress, TSN stably associates with stress granules (SGs), in a poorly understood process. Here, we show that in the model plant Arabidopsis thaliana, TSN is an intrinsically disordered protein (IDP) acting as a scaffold for a large pool of other IDPs, enriched for conserved stress granule components as well as novel or plant-specific SG-localized proteins. While approximately 30% of TSN interactors are recruited to stress granules de novo upon stress perception, 70% form a protein–protein interaction network present before the onset of stress. Finally, we demonstrate that TSN and stress granule formation promote heat-induced activation of the evolutionarily conserved energy-sensing SNF1-related protein kinase 1 (SnRK1), the plant orthologue of mammalian AMP-activated protein kinase (AMPK). Our results establish TSN as a docking platform for stress granule proteins, with an important role in stress signalling.
Abstract
European Commission 702473
Abstract
Ministerio de Economía y Competitividad IJCI-2016-30763, PGC2018-099048-B-100
Abstract
Universidad de Sevilla VIPPIT-2020-IV.4, VIPPIT-2020-I.5
Abstract
Knut and Alice Wallenberg Foundation 2018.0026
Abstract
Swedish Research Council VR 2019-04250_VR
Abstract
Swedish Foundation for Strategic Research RBP14-0037
Additional details
- URL
- https://idus.us.es/handle//11441/125556
- URN
- urn:oai:idus.us.es:11441/125556
- Origin repository
- USE