Published September 7, 2021 | Version v1
Publication

Tudor staphylococcal nuclease is a docking platform for stress granule components and is essential for SnRK1 activation in Arabidopsis

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Description

Tudor staphylococcal nuclease (TSN; also known as Tudor-SN, p100, or SND1) is a multifunctional, evolutionarily conserved regulator of gene expression, exhibiting cytoprotective activity in animals and plants and oncogenic activity in mammals. During stress, TSN stably associates with stress granules (SGs), in a poorly understood process. Here, we show that in the model plant Arabidopsis thaliana, TSN is an intrinsically disordered protein (IDP) acting as a scaffold for a large pool of other IDPs, enriched for conserved stress granule components as well as novel or plant-specific SG-localized proteins. While approximately 30% of TSN interactors are recruited to stress granules de novo upon stress perception, 70% form a protein–protein interaction network present before the onset of stress. Finally, we demonstrate that TSN and stress granule formation promote heat-induced activation of the evolutionarily conserved energy-sensing SNF1-related protein kinase 1 (SnRK1), the plant orthologue of mammalian AMP-activated protein kinase (AMPK). Our results establish TSN as a docking platform for stress granule proteins, with an important role in stress signalling.

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European Commission 702473

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Ministerio de Economía y Competitividad IJCI-2016-30763, PGC2018-099048-B-100

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Universidad de Sevilla VIPPIT-2020-IV.4, VIPPIT-2020-I.5

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Knut and Alice Wallenberg Foundation 2018.0026

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Swedish Research Council VR 2019-04250_VR

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Swedish Foundation for Strategic Research RBP14-0037

Additional details

Created:
December 5, 2022
Modified:
November 30, 2023