Cloning and Biochemical Characterization of a Class A β-Lactamase from Prevotella intermedia

Creators: Prêcheur, Isabelle; Madinier, I.; Fosse, T.; Giudicelli, J.; Labia, R.

Others:: Microbiologie Orale, Immunothérapie et Santé (MICORALIS) ; Université Nice Sophia Antipolis (1965 - 2019) (UNS) ; COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Université Côte d'Azur (UCA); Microbiologie : Risques Infectieux ; Université de Rennes 1 (UR1) ; Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-CHU Pontchaillou [Rennes]-Faculté de Chirurgie Dentaire de Rennes-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique )

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ABSTRACT The gene encoding a β-lactamase of Prevotella intermedia was cloned and sequenced. This gene, called cfxA2 , shared 98% identity with cfxA , the structural gene of a β-lactamase previously described in Bacteroides vulgatus . The deduced protein sequence had a K272E substitution. CfxA2 had the characteristics of class A, group 2e β-lactamases.

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Cloning and Biochemical Characterization of a Class A β-Lactamase from Prevotella intermedia

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