Localized induction of the ntcA regulatory gene in developing heterocysts of Anabaena sp. strain PCC 7120

Description

The ntcA gene encodes an N-control transcriptional regulator in cyanobacteria. In the N2-fixing, heterocyst-forming cyanobacterium Anabaena sp. strain PCC 7120, ntcA is an autoregulatory gene that is transcribed from a complex promoter region that includes a constitutive promoter (P 2) and promoters that are induced upon N step-down (P1 and P3). Expression of ntcA was investigated with the use of an ntcA-gfp translational fusion, which was introduced both in the natural ntcA locus and in a heterologous genomic place. Induction of ntcA-gfp took place after N step-down in all the cells of the filament, but at especially high levels in developing heterocysts. Localized induction could be driven independently by P3 and P1.

Abstract

Ministerio de Educación y Ciencia BFU2004-00872

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