Engineering heterothallic strains in fission yeast
- Others:
- Institut de biochimie et génétique cellulaires (IBGC) ; Université Bordeaux Segalen - Bordeaux 2-Centre National de la Recherche Scientifique (CNRS)
- Institut de Recherche sur le Cancer et le Vieillissement (IRCAN) ; Université Nice Sophia Antipolis (1965 - 2019) (UNS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Côte d'Azur (UniCA)
- ANR-18-CE13-0009,eVOLve,Impact du volume cellulaire sur le processus d'adaptation(2018)
Citation
Description
In poor nitrogen conditions, fission yeast cells mate, undergo meiosis and form spores that are resistant to deleterious environments. Natural isolates of Schizosaccharomyces pombe are homothallic. This allows them to naturally switch between the two h− and h+ mating types with a high frequency, thereby ensuring the presence of both mating partners in a population of cells. However, alteration of the mating type locus can abolish mating type switching or reduce it to a very low frequency. Such heterothallic strains have been isolated and are common in research laboratories due to the simplicity of their use for Mendelian genetics. In addition to the standard laboratory strains, a large collection of natural S. pombe isolates is now available, representing a powerful resource for investigating the genetic diversity and biology of fission yeast. However, most of these strains are homothallic, and only tedious or mutagenic strategies have been described to obtain heterothallic cells from a homothallic parent. Here, we describe a simple approach to generate heterothallic strains. It takes advantage of an alteration of the mating type locus that was previously identified in a mating type switching‐deficient strain and the CRISPR‐Cas9 editing tool, allowing for a one‐step engineering of heterothallic cells with high efficiency.
Abstract
International audience
Additional details
- URL
- https://hal.science/hal-04621601
- URN
- urn:oai:HAL:hal-04621601v2
- Origin repository
- UNICA