BAUTISTA , Lola

Differential-interference-contrast (DIC) microscopy is an optical microscopy technique widely used in biology to observe unstained transparent specimens, in which a two-dimensional image is formed from the interference of two waves that have a lateral differential displacement (shear) and are phase shifted relative one to each other. Following...

The DIC image is formed by the interference of two orthogonally polarized beams that have a lateral displacement (called shear) and are phase shifted relatively one to each other. The resulting image has a 3D high contrast appearance, which can be enhanced by introducing a uniform phase difference between the beams (called bias).

In the last forty years, differential-interference-contrast (DIC) microscopy has gained popularity in biomedical research as an effective optical microscopy technique used to observe unstained transparent specimens under a transmitted-light configuration. The DIC image formation is caused by the interference of two orthogonally polarized beams,...

We present a gradient-based optimization method for the estimation of a specimen's phase function from polychromatic DIC images. The method minimizes the sum of a nonlinear least-squares discrepancy measure and a smooth approximation of the total variation. A new formulation of the gradient and a recent updating rule for the choice of the step...

In this paper we address the problem of estimating the phase from color images acquired with differential–interference–contrast microscopy. In particular, we consider the nonlinear and nonconvex optimization problem obtained by regularizing a least–squares–like discrepancy term with an edge–preserving functional, given by either the...

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In this paper we address the problem of estimating the phase from color images acquired with differential-interference-contrast microscopy. In particular, we consider the nonlinear and nonconvex optimization problem obtained by regularizing a least-squares-like discrepancy term with a total variation functional, possibly smoothed with the...