A key feature of eukaryotic cells is the asymmetric distribution of lipids along their secretory pathway. Because of the biological significance of these asymmetries, it is crucial to define the mechanisms which create them. Extensive studies have led to the identification of lipid transfer proteins (LTPs) that work with lipid-synthesizing...
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March 31, 2023 (v1)Journal articleUploaded on: November 25, 2023
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March 2, 2020 (v1)Journal article
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May 3, 2010 (v1)Journal article
Numerous data have been collected on lipid-binding amphipathic helices involved in membrane-remodeling machineries and vesicular transport. Here we describe how, with regard to lipid composition, the physicochemical features of some amphipathic helices explain their ability to recognize membrane curvature or to participate in membrane...
Uploaded on: December 4, 2022 -
July 2007 (v1)Journal article
Signaling proteins are usually composed of one or more conserved structural domains. These domains are usually regulatory in nature by binding to specific activators or effectors, or species that regulate cellular location, etc. Inositol-specific mammalian phospholipase C (PLC) enzymes are multidomain proteins whose activities are controlled by...
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February 22, 2019 (v1)Book section
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January 2020 (v1)Journal article
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Uploaded on: February 22, 2023 -
May 2009 (v1)Journal article
A cellular membrane is highly deformable: during the last decade, numerous studies have dissected at the molecular scale how during vesicular transport various proteins could deform a membrane or recognize membrane deformation. We will discuss how the activity of ArfGAP1 and GMAP-210, two proteins involved in vesicular transport, is regulated...
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January 2020 (v1)Journal article
Lipids are precisely distributed in the eukaryotic cell where they help to define organelle identity and function, in addition to their structural role. Once synthesized, many lipids must be delivered to other compartments by non-vesicular routes, a process that is undertaken by proteins called Lipid Transfer Proteins (LTPs). OSBP and the...
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January 2020 (v1)Journal article
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July 28, 2008 (v1)Journal article
SUMMARY: HELIQUEST calculates the physicochemical properties and amino acid composition of an alpha -helix and screens databank to identify protein segments possessing similar features. This server is also dedicated to mutating helices manually or automatically by genetic algorithm to design analogues of defined features. AVAILABILITY:...
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July 21, 2020 (v1)Journal article
Lipids are amphiphilic molecules that self-assemble to form biological membranes. Thousands of lipid species coexist in the cell and, once combined, define organelle identity. Due to recent progress in lipidomic analysis, we now know how lipid composition is finely tuned in different subcellular regions. Along with lipid synthesis, remodeling...
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March 1, 2012 (v1)Journal article
In recent years, the interest for proteins that exert key functions in vesicular trafficking through their ability to sense or induce positive membrane curvature has expanded. In this chapter, we first present simple protocols to determine whether a protein targets positively curved membranes with liposomes of well-defined size. Next we...
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March 14, 2021 (v1)Journal article
International audience
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November 14, 2023 (v1)Publication
Lipid distribution in the eukaryotic cells depends on tight couplings between lipid transfer and lipid metabolism. Yet these couplings remain poorly described. Notably, it is unclear to what extent lipid exchangers of the OSBP-related proteins (ORPs) family, coupled to PI(4)P metabolism, contribute to the formation of sterol and...
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May 2, 2008 (v1)Journal article
Golgins, long stringlike proteins, tether cisternae and transport vesicles at the Golgi apparatus. We examined the attachment of golgin GMAP-210 to lipid membranes. GMAP-210 connected highly curved liposomes to flatter ones. This asymmetric tethering relied on motifs that sensed membrane curvature both in the N terminus of GMAP-210 and in...
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2015 (v1)Journal article
The recruitment of many cytosolic factors is regulated by the lipid composition but also by the shape of organelles membrane. Ten years ago, we identified in ArfGAP1 a motif of 30 amino-acids termed ALPS that allows this protein to detect the positive curvature of the COPI vesicles at the end of their biogenesis and to trigger the...
Uploaded on: March 25, 2023 -
February 2007 (v1)Journal article
The Golgi-associated protein ArfGAP1 has an unusual membrane-adsorbing amphipathic alpha-helix: its polar face is weakly charged, containing mainly serine and threonine residues. We show that this feature explains the specificity of ArfGAP1 for curved versus flat lipid membranes. We built an algorithm to identify other potential amphipathic...
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February 20, 2007 (v1)Journal article
ArfGAP1 (Arf GTPase activating protein 1) controls the cycling of the COPI coat on Golgi membranes by catalyzing GTP hydrolysis in the small G protein Arf1. ArfGAP1 contains a central motif named ALPS (ArfGAP1 lipid-packing sensor) that adsorbs preferentially onto highly curved membranes. This motif allows coupling of the rate of GTP hydrolysis...
Uploaded on: February 28, 2023 -
September 2, 2019 (v1)Journal article
A central assumption is that lipid transfer proteins (LTPs) bind transiently to organelle membranes to distribute lipids in the eukaryotic cell. Osh6p and Osh7p are yeast LTPs that transfer phosphatidylserine (PS) from the endoplasmic reticulum (ER) to the plasma membrane (PM) via PS/phosphatidylinositol-4-phosphate (PI4P) exchange cycles. It...
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November 22, 2021 (v1)Publication
Abstract Several members of the oxysterol-binding protein-related proteins (ORPs)/oxysterol-binding homology (Osh) family exchange phosphatidylserine (PS) and phosphatidylinositol 4-phosphate (PI(4)P) at the endoplasmic reticulum/plasma membrane (PM) interface. It is unclear whether they preferentially exchange PS and PI(4)P with specific acyl...
Uploaded on: December 3, 2022 -
November 20, 2021 (v1)Journal article
Background: Lipid species are accurately distributed in the eukaryotic cell so that organelle and plasma membranes have an adequate lipid composition to support numerous cellular functions. In the plasma membrane, a precise regulation of the level of lipids such as phosphatidylserine, PI(4)P, and PI(4,5)P 2 , is critical for maintaining the...
Uploaded on: December 3, 2022 -
November 2013 (v1)Journal article
Several proteins at endoplasmic reticulum (ER)-Golgi membrane contact sites contain a PH domain that interacts with the Golgi phosphoinositide PI(4)P, a FFAT motif that interacts with the ER protein VAP-A, and a lipid transfer domain. This architecture suggests the ability to both tether organelles and transport lipids between them. We show...
Uploaded on: December 4, 2022