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Last name: FARETTA
First name: Mario
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Stimulated emission depletion (STED) microscopy and Separation of Photons by Lifetime Tuning (SPLIT) provide optical super-resolution imaging, at the nanometer scale, on intact single cell nuclei. The resolution that can be achieved is limited by factors such as photobleaching and/or reduction of signal-to-noise, which depend on the conditions...
Oncogenes activation may lead to alterations in chromatin organization at the nanoscale by interfering with fundamental processes such as DNA transcription, DNA replication, and DNA Damage Response [1]. The in vitro observation of these molecular processes is crucial to understand how oncogenes can alter chromatin organization leading to tumor...
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The molecular mechanisms that underlie oncogene-induced genomic damage are still poorly understood. To un-derstand how oncogenes affect chromatin architecture, it is important to visualize fundamental processes such as DNA replica-tion and transcription in intact nuclei and quantify the alterations of their spatiotemporal organization induced...
Defects in the global organization of chromatin are relevant in many pathological processes, including cancer. The activation of oncogenes has been recognized as a triggering event of alterations in the dynamics of replication, transcription and DNA damage response (DDR), that may lead to increased DNA damage and the onset of genomic...
The possibility of integrating different light-matter interaction ways for producing images in optical microscopy is the starting point for the design and realization of a brand new optical microscope that has been named "liquid tunable microscope". This represents a new paradigm in data collection and image formation with a potential high...
The fact that the size of many molecular complexes is in the range of 10 to 100nm has generated a growing interest for the development of super-resolved fluorescence microscopy techniques. Optical super-resolution is generally achieved by switching on-off the fluorophores by stochastic or targeted approaches [1]. For instance, in stimulated...