Take away points:-Conventional methods for obtaining heterothallic fission yeast strains are inefficient-We implemented a streamlined genetic editing approach to engineer heterothallism-All fission yeast isolates reported in Jeffares et al. 2015 can be engineered-This method enhances the exploration of genetic diversity in wild fission yeast
-
October 19, 2023 (v1)PublicationUploaded on: November 25, 2023
-
December 15, 2023 (v1)Journal article
In poor nitrogen conditions, fission yeast cells mate, undergo meiosis and form spores that are resistant to deleterious environments. Natural isolates of Schizosaccharomyces pombe are homothallic. This allows them to naturally switch between the two h− and h+ mating types with a high frequency, thereby ensuring the presence of both mating...
Uploaded on: October 29, 2024