DNA double-strand breaks (DSBs) represent the most cytotoxic DNA lesion that can arise from either endogenous and exogenous genotoxic stresses. There are two major and alternative pathways to repair DSBs: non-homologous endjoining (NHEJ) and homologous recombination (HR). The choice between these repair mechanisms is highly regulated in order...
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June 14, 2018 (v1)PublicationUploaded on: December 5, 2022
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February 8, 2019 (v1)Publication
DNA-end resection is a highly regulated and critical step in the response and repair of DNA double-strand breaks. In higher eukaryotes, CtIP regulates resection by integrating cellular signals via its posttranslational modifications and protein-protein interactions, including cell-cycle-controlled interaction with BRCA1. The role of BRCA1 in...
Uploaded on: March 27, 2023 -
March 2, 2018 (v1)Publication
While regulating the choice between homologous recombination and non-homologous end joining (NHEJ) as mechanisms of double-strand break (DSB) repair is exerted at several steps, the key step is DNA end resection, which in Saccharomyces cerevisiae is controlled by the MRX complex and the Sgs1 DNA helicase or the Sae2 and Exo1 nucleases. To assay...
Uploaded on: December 5, 2022 -
March 17, 2017 (v1)Publication
There are two major and alternative pathways to repair DNA double-strand breaks: non-homologous end-joining and homologous recombination. Here we identify and characterize novel factors involved in choosing between these pathways; in this study we took advantage of the SeeSaw Reporter, in which the repair of double-strand breaks by...
Uploaded on: March 27, 2023