LOPES , Filipa

The production of microalgae using biofilm-based processes is becoming popular because of their higher productivity compared to traditional culture systems. Another advantage of microalgal biofilms is the straightforward harvesting procedure achieved by scraping off the biofilm, significantly reducing the energy demand required when...

Determining the fraction of viable cells in algal cultures is critical to improve the understanding and control of algal microbiology, ecology, and biotechnology. Whereas current techniques for algal viability determination can be rather cumbersome, this paper describes a new assay that enables the rapid quantification of algal viability using...

Microalgae biofilms have been proposed as an efficient alternative to suspended cultures [1]. They offerenhanced productivities and straightforward harvesting by simple scrapping. However, little is known abouttheir structure (spatial arrangement of cells, colonies and Extracellular PolymerS; EPS), which maystrongly impact bioprocess stability...

Microalgae biofilms have been proposed as an alternative to suspended cultures in 10 commercial and biotechnological fields. However, little is known about their architecture which 11 may strongly impact biofilm behavior, bioprocess stability and productivity. In order to unravel the 12 architecture of microalgae biofilms, four species of...

We present a spatial model describing the growth of a photosynthetic microalgae biofilm. In this 2D-model we consider photosynthesis, cell carbon accumulation, extracellular matrix excretion, and mortality. The rate of each of these mechanisms is given by kinetic laws regulated by light, nitrate, oxygen and inorganic carbon. The model is based...

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Introduction Biofilm-based microalgae production technologies offer enormous potential for improving sustainability and productivity. However, the light pattern induced by these technologies is a key concern for optimization. Methods In this work, the effects of light/dark cycles on architecture, growth, and physiology of Chlorella vulgaris...

Abstract Biofilm-based microalgae technology improves productivity, reduces energy consumption and facilitates harvesting. However, the effect of light received less attention than for planktonic cultures. This work assessed the effect of Photon Flux Density (PFD) on Chlorella vulgaris biofilm dynamics (structure, physiology, activity)....

The interest by biofilm-based microalgae technologies has increased lately due to productivity improvement, energy consumption reduction and easy harvesting. However, the effect of light, one key factor for system's operation, received less attention than for planktonic cultures. This work assessed the impact of Photon Flux Density (PFD) on...

The ability of microalgae to fix carbon dioxide and convert it into biofuels, foods and other valuable products has drawn a lot of scientific attention in the last decades. In the last years a number of works aimed at understanding the influence of daily and seasonal temperature fluctuations that affect cell metabolism, and thus biomass...

Microalgae biofilms have a great ecological importance and a high biotechnological potential. Nevertheless, an in-depthand combined structural (i.e. the architecture of the biofilm) andphysiological characterization of microalgaebiofilmsis still missing.An approach able to provide at the same time physiological and structuralinformation during...

The architecture of microalgae biofilms has been poorly investigated, in particular with respect toshear stress, which is a crucial factor in biofilm-based reactor design and operation. To investigatehow microalgae biofilms respond to different hydrodynamic regimes, the architecture and cohesionof Chlorella vulgaris biofilms were studied in...

Microalgae biofilms have a great ecological importance and a high biotechnological potential. Nevertheless, an in-depth and combined structural (i.e. the architecture of the biofilm) and physiological characterization of microalgae biofilms is still missing. An approach able to provide at the same time physiological and structural information...

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Abstract Microalgae biofilm emerged as a solid alternative to conventional suspended cultures which present high operative costs and complex harvesting processes. Among several designs, rotating biofilm‐based systems stand out for their scalability, although their primary applications have been in wastewater treatment and aquaculture. In this...

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Biofilm-based systems have gained increasing attention for microalgae cultivation dueto their potential to improve productivity, reduce water and energy demands andeventually operating costs. However, effective operation of these systems requiresprocess control, which relies on the development of on-line monitoring of processvariables such as...

Biofilm-based systems have gained increasing attention for microalgae cultivation due to their potential to improve productivity, decrease water and energy demands and eventually lower operating costs. The effective operation of these systems is based on process control, which requires on-line monitoring of process variables such as biomass...

Microalgae Biofilm-based Cultivation Systems (MBCS) are emerging as a promising solutionfor microalgae production, offering increased productivity, simplified harvesting, and loweroperating costs. However, efficient monitoring routines for MBCS are still lacking, andcurrent approaches involve invasive and destructive sampling, which emphasizes...

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